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产品目录

Bme18I内切酶图片
产品货号:
GS2076
中文名称:
Bme18I内切酶
英文名称:
Bme18I
产品规格:
500U
发货周期:
1~3天
产品价格:
询价
储存条件:冷冻(-20℃)

技术规格

Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sitesG↑GWCC
来源Bacillus megaterium 18
Assayed onLambda DNA
Optimal bufferO(50mMTris-HCl(pH 7.6 at 25℃);10 mM MgCl2;100 mM NaCl;1 mM DTT.)
Optimal temperature37℃
Storage conditions10 mM Tris-HCl(pH 7.5);100 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;100 μg/ml BSA;50%glycerol.Store at -20℃.
LigationsAfter 10-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 37℃.
Methlation effectCleaved of DNA is impaired by overlapping dcm-methylation(CmCWGG):GGWCCWGG.
Thermal inactivationenzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible EndsAflI,AvaII,Eco47I,PspO3I^,SinI,Vpak11AI^

相关搜索:Bme18I内切酶Bme18I限制性内切酶
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