产品货号：
GS2076
中文名称：
Bme18I内切酶
英文名称：
Bme18I
产品规格：
500U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：Bme18I内切酶，Bme18I限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | G↑GWCC |
| 来源 | Bacillus megaterium 18 |
| Assayed on | Lambda DNA |
| Optimal buffer | O(50mMTris-HCl(pH 7.6 at 25℃);10 mM MgCl2;100 mM NaCl;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);100 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;100 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After 10-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Cleaved of DNA is impaired by overlapping dcm-methylation(CmCWGG):GGWCCWGG. |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | AflI,AvaII,Eco47I,PspO3I^,SinI,Vpak11AI^ |
相关搜索：Bme18I内切酶，Bme18I限制性内切酶